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Fluorescence imaging, at macro, micro and sub-micro scales, has revolutionized biological science in the past 30 years. Immuno-labeling has provided the precise targeting of molecules in the fixed tissue, while fluorescent proteins have enabled localization in living tissues. Fluorescent indicators enable the imaging of dynamic changes in cell metabolism.
This book covers for the first time imaging at all scales, from macro to sub-micro (super-resolution). The authors include Robert Clegg, legendary teacher and researcher (who sadly passed away during the editorial process); Jim Pawley, editor of several editions of the Handbook of Biological Confocal Microscopy; the famous and now dispersed New Zealand team of Mark Cannell, Christian Soeller, and David Baddeley; Robert Hoffman, pioneer of whole-animal imaging in cancer research; Andreas Schoenle and Christian Eggeling, experts in STED nanoscopy; and many other famous participants in this field. All the contributors are at the cutting edge of their field.