:

product description page

Low-cost Methods for Molecular Characterization of Mutant Plants : Tissue Desiccation, DNA Extraction

Low-cost Methods for Molecular Characterization of Mutant Plants : Tissue Desiccation, DNA Extraction - image 1 of 1

about this item

This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.

Edition: Reprint
Number of Pages: 45
Genre: Science
Format: Paperback
Publisher: Springer Verlag
Author: Bradley J. Till & Joanna Jankowicz-cieslak & Owen A. Huynh & Mayada M. Beshir & Robert G. Laport
Language: English
Street Date: November 4, 2016
TCIN: 51969825
UPC: 9783319386676
Item Number (DPCI): 248-37-7207

guest reviews

Prices, promotions, styles and availability may vary by store & online. See our price match guarantee. See how a store is chosen for you.