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Methods in Yeast Genetics and Genomics 2015 : A Cold Spring Harbor Laboratory Course Manual (Hardcover)

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About this item

This updated edition of the Cold Spring Harbor Laboratory course manual provides eleven teaching experiments and protocols, and recipes for the standard techniques and reagents used in the study of yeast Saccharomyces cerevisiae as a model of multiple cellular processes. The experiments emphasize combinations of classical and modern genetic approaches, including the isolation and characterization of mutants, two-hybrid analysis, tetrad analysis, complementation, and recombination, as well as molecular genetic techniques like yeast transformation, mating-type switching, gene replacement by polymerase chain reaction, construction and analysis of gene fusions, and generation of mutations. They also introduce fundamental techniques in yeast genomics, such as performance and interpretation of multiplexed sequencing and comparative genome hybridization to DNA arrays, in addition to comparative genomics using different yeast strains to study natural, variation, evolution, and quantitative traits, and modern cytological approaches like epitope tagging and imaging yeast cells using green fluorescent protein-protein fusions and fusion indicators for subcellular organelles and transcriptional readouts. Annotation ©2016 Ringgold, Inc., Portland, OR (protoview.com)
Number of Pages: 233
Genre: Science
Sub-Genre: Genetics
Format: Hardcover
Publisher: Cold Spring Harbor Laboratory Pr
Author: Maitreya J. Dunham & Marc R. Gartenberg & Grant W. Brown
Language: English
Street Date: August 26, 2015
TCIN: 50107805
UPC: 9781621821342
Item Number (DPCI): 248-00-6803
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