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Tunability has added an important dimension to a variety of laser applications and has led to new laser systems. From laser spectroscopy to Bose-Einstein condensation, the tunable laser is needed. This new edition reflects significant developments and describes the physics and architecture of widely applied tunable laser sources, emphasizing biomedical applications of fiber lasers and ultrashort pulsed lasers as well as isotope separation and cancer photodynamic therapy. The new edition also expands on Quantitative Lithium and Rubidium Diode Laser Spectroscopy.