Immunohistochemistry and Immunocytochemistry - (Essential Methods) 2nd Edition by Simon Renshaw (Paperback)

Book Synopsis

Immunohistochemistry and immunocytochemistry are invaluable tools for the visualization of tissue and cellular antigens in diagnostic and biological research environments. The need to obtain accurate, reliable and reproducible results is paramount.

It is with this fundamental aim in mind that we have compiled Immunohistochemistry: Essential Methods. We have achieved this by examining each aspect of immunochemistry in turn, with each chapter including detailed information regarding the subject matter in question. Each chapter is written by an expert in their field and includes protocols that are typically used in their own research. Subjects covered are, amongst others, antibodies and their production; selection of reporter labels; immunochemical staining methods and experimental design (both using single and multiple reporter labels); quality assurance; automated immunochemistry; confocal microscopy and electron microscopy. In addition, benefits and limitations of each approach are discussed within the chapters.

From the Back Cover

Immunohistochemistry and immunocytochemistry are invaluable tools for the visualization of tissue and cellular antigens in diagnostic and biological research environments. The need to obtain accurate, reliable and reproducible results is paramount.

Immunohistochemistry and Immunocytochemistry: Essential Methods is the second edition of Immunohistochemisrty: Methods Express, previously published by Scion Publishing. The book is a comprehensive research guide that describes the methodologies of immunochemistry. Each chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good theoretical information and practical advice, for immediate use at the bench. Subjects covered include, antibodies and their production; selection of reporter labels; immunochemical staining methods and experimental design (both using single and multiple reporter labels); quality assurance; automated immunochemistry; confocal microscopy and electron microscopy.

This book offers a wealth of knowledge to the novice immunochemist, who wishes to understand fully the theory and practice of immunochemical staining techniques and obtain reliable and reproducible data time and time again. For the experienced immunochemist, it is a comprehensive reference guide, allowing optimization of existing immunochemical staining protocols and a resource to further increase knowledge and understanding.

About the Author

Simon Renshaw is the editor of Immunohistochemistry and Immunocytochemistry: Essential Methods, 2nd Edition, published by Wiley.