DNA Damage and Chromosomal Instability - (Methods in Cell Biology) (Hardcover)

Book Synopsis

DNA Damage and Chromosomal Instability, Volume 182 in the Methods in Cell Biology series, presents interesting chapters on a variety of timely topics, including Induction of chromosome-specific micronuclei and chromothripsis by centromere inactivation, Generation of aneuploid cells and assessment of their ability to survive in presence of chemotherapeutic agents, Genetic reporters to detect and quantify homologous recombination in yeast, Mapping histone variant genomic distribution: exploiting SNAP- Tag labelling to follow the dynamics of incorporation of H3 variants, Enrichment of DNA replication intermediates by EdU pull down, Nascent DNA sequencing and its diverse applications in genome integrity research, and much more. Additional chapters cover Enrichment of DNA replication intermediates by EdU pull down, DNA curtains for studying phase separation mechanisms of DNA-organizing proteins, Visualizing the Dynamics of DNA Replication and Repair at the Single-Molecule Molecule Level, Cloning and Expansion of Repetitive DNA Sequences, Using an ImageJ-based script to detect replication stress and associated cell cycle exit from G2 phase by fluorescence microscopy Arne Lindqvist

Review Quotes

"...addresses experimental methods for inducing DNA damage and chromosome instability, methods for evaluating the mechanisms of causation, and approaches to the study of the consequences of these events. It also explores mechanisms of repair of DNA and chromosome damage, ... the cumulative work of 55 authors.... [H]ighly technically focused, ... each chapter begins with a short abstract followed by introductory and background information. Detailed laboratory methods follow the introduction, and these include step-by-step instructions for the procedures discussed.... [U]niquely easy to use for active researchers . Overall, this volume accomplishes what it sets out to do quite well. The detailed laboratory methods presented in each chapter are a strong point of the book." --(c)Doody's Review Service, 2024, Mark F. Sanders, PhD (UC Davis College of Biological Sciences)