In Situ Molecular Pathology and Co-Expression Analyses - 2nd Edition by Gerard J Nuovo (Paperback)

Book Synopsis

Major advancements in the field of in situ molecular pathology have occurred since publication of the first edition. In Situ Molecular Pathology and Co-expression Analyses, Second Edition, continues to teach both the molecular basis for the improvements and the actual protocols. This is the unique feature that separates it from the pack of other "cook-book" type approaches. The fields of in situ hybridization and immunohistochemistry have expanded rapidly where computer-based analyses systems have greatly expanded the power of these methods. Further, knowledge of the marked improvements in the reagents themselves since the first edition can make the difference of excellent versus misleading data. The automated platforms require that researchers and diagnostic biomedical investigators have a good understanding of the basics of in situ based tests, protocols, and biochemistry for troubleshooting in order to maximize the use of these platforms. This second edition focuses attention on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses. Practicing molecular pathologists, diagnostic pathologists, laboratory directors, and toxicologists, as well as clinicians and researchers in training, will benefit from this clear presentation of protocols and theoretical framework. Data derived from in situ hybridization and immunohistochemistry.

Review Quotes

Praise for the First Edition: "If you perform this type of testing in your laboratory, you must get this book. When you think all experimental approaches for in situ molecular testing methods have failed, have faith and refer to this book for the solution." --Doody.com, 2013

"Nuovo presents a textbook on in-situ-based molecular pathology for readers with minimum prior knowledge of molecular pathology in general and in-situ-based molecular pathology in particular." --Reference and Research Book News, 2013

*4 stars* "...explain[s] the theory and foundation of IHC and ISH and present[s] easy-to follow experimental protocols with "tricks of the trade.".... [W]ritten for practicing surgical pathologists, molecular pathologists, laboratory directors and toxicologists, as well as clinicians and researchers in training.... [A]ccompanied by good numbers of microphotographs of very good quality, as well as illustrations and graphs when pertinent. Each chapter contains a comprehensive list of references, [and] a useful index is also provided.... [A]n excellent resource to learn and understand the techniques of IHC and ISH and their application in diagnostic and research settings.... [C]ontains all the information on IHC and ISH that an average pathologist or researcher would ever need, and I highly recommend it." --(c)Doody's Review Service, 2022, Anamarija Perry, MD (University of Michigan Medical School)